Microbiome-metabolome interactions

Dental plaque is a biofilm and is an important indicator of oral health. When left untreated, it can lead to inflammation of the gums, also known as gingivitis. A biofilm is a local organization of microbes that is attached to a surface – in this case the tooth surface. The exact mechanics that allow for the formation of dental plaque are unclear.

In this project, we analyze the Top Institute of Food and Nutrition dataset, which follows a cohort of individuals during a period of two weeks where they are not allowed to brush their teeth. Oral microbiome samples are taken at six places in the mouth at various timepoints, also before and after the intervention. Oral metabolomics samples are taken from unstimulated saliva only during the intervention period.

We aim to elucidate the complex interaction between microbiome and metabolome using Parallel Factor Analysis. This method is able to describe the bits in the data where the variation is highest, also over time. By comparing the models, we can form hypotheses on the microbiome-metabolome interactions that occur.

Scientists involved in this project

Roel van der Ploeg
Anna Heintz-Buschart
Johan Westerhuis
Age Smilde
Egija Zaura (ACTA)
Monique van der Veen (ACTA)
Bart Keijser (TNO)

In coorporation with

Acadamsich Centrum Tandheelkunde Amsterdam (ACTA)
Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek (TNO)